N-to-C sequential ligation using peptidyl N,N-bis(2-mercaptoethyl)amide building blocks |
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Authors: | Yang Renliang Hou Wen Zhang Xiaohong Liu Chuan-Fa |
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Institution: | Structural Biology and Biochemistry Division, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. |
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Abstract: | A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin. |
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