Change of concentrations of trace elements and protein contents in the liver of zinc deficient mice |
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| Authors: | R. Minayoshi T. Ohyama N. Kinugawa J. Kamishima T. Ogi K. Ishikawa M. Noguchi H. Suganuma K. Takahashi S. Enomoto M. Yanaga |
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| Affiliation: | (1) Radiochemistry Research Laboratory, Faculty of Science, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;(2) Department of Biology and Geosciences, Faculty of Science, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;(3) Cycrotlon Center, RIKEN, The Institute of Physical Chemical Research, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan |
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| Abstract: | The concentrations of essential trace elements and proteins in cytosolic fraction of hepatic cells of mice fed with Zn-deficient diet (Zn-def, mice) and control were determined by ICP-MS and BCA protein assay method, respectively, after division into forty fractions by gel filtration chromatography. The concentrations of zinc and proteins decreased in the 14–17th fractions of Zn-def, mice, whereas cobalt concentrations increased in the 14, 17, and 18th fractions. However, no significant differences were found on the gel after SDS-PAGE for the 12–21st fractions, although the BCA protein assay date showed the decrease of protein amounts in 13–15th fractions of Zn-def. mice. |
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