Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers |
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Authors: | Carmon G Fishov I Feingold M |
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Institution: | Department of Physics, Ben-Gurion University of the Negev, Beer Sheva, Israel. |
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Abstract: | Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments. |
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