一种新型手性配体交换色谱键合固定相

采用气相或液相色谱法拆分氨基酸对映体通常一化过程，费时而麻烦，而且有可能发生消旋，影响分析结果，手性配体交换色谱法是拆分氨基酸和羟基酸对映体的一种有效方法，其选择性高，不需柱前衍生。Davankov等对此方法进行了评述。此法大都采用刚性环状结构的光活性脯氨酸或羟基脯氨酸键合相作为柱填料，Jeanneret-Gris等曾采用1，2，3，4－四氢－3－异喹啉羧酸接枝的聚丙烯酰胺相拆分氨基酸对映体。

A Novel Bonded Stationary Phase for Chiral Ligand Exchange-Chromatography

A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding (S)-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid prepared from L-Phe to YWG-80 silica gel via 3-glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL-amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C2H5)3, 2 mmol/L HAc and 0.2 mmol/L Cu(Ac)2 as the mobile phase with a flow rate 1.0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL-amino acids on the CBSP was found to be between 1.11 and 1.51. The elution order of D-isomer before L-isomer on the CBSP was observed for all the DL-amino acids resolved except DL-Val. For DL-Pro, DL-Val and DL-Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with a five-membered ring structure.