近红外光谱在蛋白质和含酰胺基团聚合物研究中的应用*

近红外光谱(near-infrared spectroscopy，NIR)是一种常用的无损表征手段，但谱带强度弱、交叠情况严重等缺点局限了它的应用范围。本文介绍了几种常见的改善近红外光谱技术不足的方法，如二阶导数法、二维相关光谱法和化学计量法等，并举例阐述了近红外光谱在蛋白质和含酰胺基团聚合物的结构和含量等方面的应用。这些方法对近红外光谱的定性定量分析起了很好的辅助作用，有效地拓宽了近红外光谱技术的应用领域。

Application of Near-infrared Spectroscopy in the Study of Protein and Polymer with Amide Group

Near-infrared spectroscopy is a widely-used nondestructive analytical tool. But the disadvantages of weak band intensities and greatly overlapped bands have limited its application. In this review, several methods to improve the drawbacks of NIR spectroscopy, such as second-derivative spectrum, two-dimensional correlation spectroscopy and chemometrics, are introduced. The applications of NIR spectroscopy after the useful treatments mentioned here in the studies of the structures and contents of both proteins and polymers with amide groups are discussed. All these show that the methods do a lot of help in the qualitative and quantitative analysis of near-infrared spectra and broaden the potential application of NIR spectroscopy.