| 稀土元素对3'-腺嘌呤及3'-鸟嘌呤核苷酸的水解断裂作用 |
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| 引用本文： | 朱兵,李新民,吴亦洁,赵大庆,倪嘉缵.稀土元素对3'-腺嘌呤及3'-鸟嘌呤核苷酸的水解断裂作用[J].化学学报,1998,56(1):47-51. |
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| 作者姓名： | 朱兵 李新民 吴亦洁 赵大庆 倪嘉缵 |
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| 作者单位： | 中国科学院长春应用化学研究所;中国科学院稀土化学与物理开放实验室 |
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| 基金项目： | 国家自然科学基金，中国科学院基础局资助项目(29631010) |
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| 摘 要： | 用核磁共振(NMR)波谱、HPLC和化学定磷法研究了稀土元素对3'-腺嘌呤核苷酸(3'-AMP)3及3'-鸟嘌呤核苷酸(3'-GMP)的水解断裂作用, 结果表明: 不同稀土元素对3'位核苷酸断裂有较强的特异性, 在37℃, pH9的条件下, 使3'-AMP断裂为腺嘌呤核苷(A)及无机磷, 使3'-GMP断裂为鸟嘌呤核苷(G)及无机磷, 其断裂作用远大于5'位核苷酸, 并对其水解断裂进行了研究。
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| 关 键 词： | 水解 核磁共振谱法 鸟嘌呤 腺嘌呤 核苷酸 |
| 修稿时间： | 1996年7月29日 |
Hydrolytic cleavage of adenosine-3'-monophosphate and Guanosine-3'-monophosphate by Lanthanides |
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| ZHU Bing LI Xin-Min WU Yi-Jie ZHAO Da-Qing NI Jia-Zuan.Hydrolytic cleavage of adenosine-3'-monophosphate and Guanosine-3'-monophosphate by Lanthanides[J].Acta Chimica Sinica,1998,56(1):47-51. |
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| Authors: | ZHU Bing LI Xin-Min WU Yi-Jie ZHAO Da-Qing NI Jia-Zuan |
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| Abstract: | The hydrolytic cleavage of adenosine-3'-monophosphate (3'-AMP) and guanosine-3'-monophosphate (3-GMP) by lanthanides was investigated by NMR, HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3'-mononucleotides. 3'-AMP and 3'-GMP were converted to Adenosine(A), phosphate and Guanosine(G), phosphate respectively at pH 9.37℃. The efficiency of cleavage was greater than that of 5'-mononucleotides. The mechanism of hydrolytic of cleavage was discussed in detail. |
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| Keywords: | lanthanide adenosine - 3' - monophosphate guanosine - 3' - monophosphate hydrolytic cleavage |
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