Visible spectrophotometric and first-derivative UV spectrophotometric determination of rifampicin and isoniazid in pharmaceutical preparations |
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Authors: | Benetton S A Kedor-Hackmann E R Santoro M I Borges V M |
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Institution: | Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de S?o Paulo, Caixa Postal 66355, CEP 05389-970, S?o Paulo, Brazil. |
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Abstract: | Two methods are described for the determination of rifampicin and isoniazid in mixtures by visible spectrophotometry and first-derivative ultraviolet spectrophotometry. The absorbance at 475 nm in buffer solution pH 7.4 was employed to determine rifampicin after applying the three-point correction technique between 420 and 520 nm, while the amplitude of the first-derivative spectrophotometric spectrum at 257 nm in HCl 0.012 M was selected for the determination of isoniazid. The methods are rapid, simple and do not require any separation step. The recovery average was 99.03% for rifampicin and 100.01% for isoniazid. The methods were applied to determine the two compounds in commercial capsules and compared with the official method of the USP XXIII with good agreement between the results. |
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