Electrochemical biosensor for detecting DNA sequences from the pathogenic protozoan Cryptosporidium parvum |
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Authors: | Wang J Rivas G Parrado C Cai X Flair M N |
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Affiliation: | Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. |
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Abstract: | An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric transduction mode for monitoring the hybridization event. Variables of the probe-immobilization, hybridization and indicator-detection steps are optimized to offer convenient quantitation of ng ml(-1) levels of the Cryptosporidium DNA target, in connection with short (3-15 min) hybridization times. The suitability for direct detection of the spiked Cryptosporidium DNA target in untreated drinking and river water samples is demonstrated. Similar performance characteristics are observed at DNA-coated microfabricated thick-film carbon strips. This and similar developments hold great promise for field screening of Cryptosporidium and other microorganisms in environmental samples. |
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