基于多壁碳纳米管修饰电极的保泰松的电致化学发光检测

基于保泰松对联吡啶钌的电致化学发光信号有较强的增敏作用, 使用多壁碳纳米管修饰玻碳电极作为工作电极, 建立了一种保泰松的电致化学发光检测方法. 对测定条件进行了一系列优化, 结果表明, 当发光试剂联吡啶钌的浓度为1.0×10－5 mol/L时, 在0.05 mol/L pH 10.20 的Na2HPO4-NaOH介质中保泰松对联吡啶钌电化学发光信号的增敏效果最强. 在此条件下, 保泰松的线性范围为0.5～200 μmol/L, 检测限(S/N＝3)为0.2 μmol/L, 与使用未经修饰的裸玻碳电极相比, 检测限下降了约一个数量级. 该检测方法还被用于加标人血清样品中保泰松的测定, 回收率在85.3%～95.0%之间, 结果令人满意, 可望用于保泰松的药物临床分析与质量监控.

Electrochemiluminescence Determination of Phenylbutazone Based on Modified Electrode by Multi-wall Carbon Nanotube

Phenylbutazone (PB) was found to be able to enhance the electrochemiluminescence (ECL) of system, based on which, a novel ECL method for the determination of PB at a multi-wall carbon nanotube (MWNT) modified glassy carbon electrode (GCE) was developed. The effect of enhanced ECL by PB was highest in the 0.05 mol/L pH 10.20 Na2HPO4-NaOH buffer with 1.0×10－5 mol/L . Under the optimum conditions, the enhanced ECL intensity was linear with PB concentration in the range of 0.5～200 μmol/L. The detection limit (defined as S/N＝3) was 0.2 μmol/L, which is lower than that on a bare GCE by one order of magnitude. The method was successfully applied to the determination of PB in a spiked human serum sample, and the results were satisfactory with the recoveries of 85.3%～95.0%. The developed detection method for PB is hopeful to be applied to clinical drug analysis and quality control.