Optimization of supercritical fluid extraction of geniposidic acid from plantain seeds using response surface methodology |
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| Authors: | Jiadan Wang Yiling Chang |
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| Institution: | 1. Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo, PR China;2. Department of Pharmaceutics, Zhejiang Pharmaceutical College, Ningbo, PR China |
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| Abstract: | The process parameters of supercritical CO2 (SCCO2) plus modifer for the extraction of geniposidic acid from plantain seeds were studied using a Box–Behnken design. The effects of independent variables, that is, ethanol concentration (0–70%, ethanol:water, v/v), extraction pressure (10–30 MPa), and temperature (50–80°C) on the yield of geniposidic acid were evaluated. Results indicated that the data could be well fitted to a second-order polynomial model. The effects of ethanol concentration and temperature, as well as the interaction between ethanol concentration and temperature were significant (p < 0.05). The yield (8.9 mg/g) of modified SCCO2 extraction at optimal conditions was compared with that obtained by Soxhlet extraction or ultrasound assisted extraction. |
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| Keywords: | geniposidic acid supercritical fluid extraction Box–Behnken design optimization plantain seeds |
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