Purification and Characterization of β-Glucosidase from a Newly Isolated Strain Tolypocladium cylindrosporum Syzx4 |
| |
基金项目: | Supported by the Important Agriculture Program of the Jilin Province Technology Department,China(No.20096013); the Jilin University Basic Science Research Fund,China(No.200903259); the Graduate Innovation Fund of Jilin University,China (No.20101043); the Project of Jilin Fuel Alcohol Company Ltd.,China |
| |
摘 要: | A higher β-glucosidase-producing strain was isolated and identified as Tolypocladium cylindrosporum syzx4 based on its morphology and internal transcribed spacer(ITS) rDNA gene sequence.The present study is to ferment,purify and characterize a β-glucosidase from T.cylindrosporum gams.The enzyme was purified to homogeneity by sulfate precipitataion,diethylaminoethyl cellulose anion exchange chromatography and Sephadex G-100 gel filtration with a 9.47-fold increase in specific activity and a recovery of 12.27...
|
关 键 词: | β-Glucosidase Purification Characterization Thermoacidophilic Tolypocladium cylindrosporum syzx4 |
本文献已被 CNKI 等数据库收录! |