| Purification and Characterization of β-Glucosidase from a Newly Isolated Strain Tolypocladium cylindrosporum Syzx4 |
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| 基金项目： | Supported by the Important Agriculture Program of the Jilin Province Technology Department,China(No.20096013); the Jilin University Basic Science Research Fund,China(No.200903259); the Graduate Innovation Fund of Jilin University,China (No.20101043); the Project of Jilin Fuel Alcohol Company Ltd.,China |
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| 摘 要： | A higher β-glucosidase-producing strain was isolated and identified as Tolypocladium cylindrosporum syzx4 based on its morphology and internal transcribed spacer(ITS) rDNA gene sequence.The present study is to ferment,purify and characterize a β-glucosidase from T.cylindrosporum gams.The enzyme was purified to homogeneity by sulfate precipitataion,diethylaminoethyl cellulose anion exchange chromatography and Sephadex G-100 gel filtration with a 9.47-fold increase in specific activity and a recovery of 12.27...
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| 关 键 词： | β-Glucosidase Purification Characterization Thermoacidophilic Tolypocladium cylindrosporum syzx4 |
Purification and Characterization ofβ-Glucosidase from a Newly Isolated Strain Tolypocladium cylindrosporum Syzx4 |
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| Authors: | ZHANG Yi-bo YUAN Li-jia CHEN Zhao-jie FU Lu LU Jia-hui MENG Qing-fan HE Huan YU Xiao-xiao LIN Feng TENG Li-rong Key Laboratory for Molecular Enzymology & Engineering Ministry of Education College of Life Science Jilin University Changchun PRChina |
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| Institution: | ZHANG Yi-bo1,YUAN Li-jia1,CHEN Zhao-jie2,FU Lu1,LU Jia-hui1,MENG Qing-fan1,HE Huan1,YU Xiao-xiao1,LIN Feng1 and TENG Li-rong1 1.Key Laboratory for Molecular Enzymology & Engineering,Ministry of Education,College of Life Science,Jilin University,Changchun 130012,P.R.China,2.Department of Endocrinology,Chian-Japan Union Hospital of Jilin University,Changchun 130033 |
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