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Immunofluorescent Labeling of Human HepG2 Cells with CdTe Quantum Dot Probe Conjugated with Anti-pan CK MAb |
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| Authors: | SUI Yu-jie ZHANG Gui-zhen WANG Qian WANG Ya-li WU Mei DU Zhen-wu ZHANG Jie JIANG Ri-hua Central Laboratory China-Japan Union Hospital of Jilin University Changchun P R China Central Laboratory the Second Hospital of Jilin University Changchun |
| Institution: | SUI Yu-jie1,2,ZHANG Gui-zhen1,WANG Qian3,WANG Ya-li1,WU Mei1,DU Zhen-wu1,ZHANG Jie4 and JIANG Ri-hua1* 1. Central Laboratory,China-Japan Union Hospital of Jilin University,Changchun 130033,P. R. China,2. Central Laboratory,the Second Hospital of Jilin University,Changchun 130041,3. Department of Urinary Surgery,4. College of Chemistry,Jilin University,Changchun 130012 |
| Abstract: | A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots(CdTe QDs) was described. Water soluble CdTe QDs were conjugated to anti-pan-cytokeratin(CK) monoclonal antibody(MAb) through coupling reagent 1-ethyl-3-(3-dimethyla- mino propyl)carbodiimide, EDC] and the conjugates were purified by dialysis. The expression of pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluoresce... |
| Keywords: | Quantum dot Pan-cytokeratin Immunofluorescence labeling Fluorescence photostability |
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