Fusion Wheat Histone H4 Protein Increases Transfection Efficiency of Non-viral DNA Vector |
| |
Authors: | WANG Chun-yan ZHANG Yu-jing College of Animal Science Veterinary Medicine Jilin University Changchun P. R. China |
| |
Affiliation: | WANG Chun-yan and ZHANG Yu-jing College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,P. R. China |
| |
Abstract: | The lack of efficient and non-toxic gene delivery, preferably with non-viral DNA vectors, is generally regarded as a major limitation for gene therapy. In this study, a wheat histone H4 gene was cloned from Triticum aestivum, sequenced, modified and expressed in E. coli. The wheat histone H4 gene and reconstructed H4TL gene encoded wheat histone H4 and a recombinant protein of 141 amino acids with an approximate molecular weight of 15500. Gel electrophoresis mobility shift assays demonstrated that the purif... |
| |
Keywords: | Wheat histone H4 Non-viral gene delivery Histonefection |
本文献已被 CNKI 等数据库收录! |
|