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Activity and Tissue Expression of Tyrosine Phosphatase PTP-MEG2 |
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| Authors: | DONG Hong-bo LI Guo-dong WANG Shao-feng FU Xue-qi ZHAO Zhi-zhuang Joe . Edmond H. Fischer Signal Transduction Laboratory College of Life Science Jilin University Changchun P. R. China . School of Pharmaceutical Science Changchun |
| Affiliation: | DONG Hong-bo1,2,LI Guo-dong3,WANG Shao-feng1,4,FU Xue-qi1* and ZHAO Zhi-zhuang Joe1,4* 1. Edmond H. Fischer Signal Transduction Laboratory,College of Life Science,Jilin University,Changchun 130012,P. R. China,2. School of Pharmaceutical Science,Changchun 130021,3. The Second Hospital of Jilin University,Changchun 130041,4. Department of Pathology,University of Oklahoma Health Sciences Center,Oklahoma City,Oklahoma 73104,USA |
| Abstract: | Protein tyrosine phosphatases(PTPs) are crucial regulators of signal transduction. Among them, PTP-MEG2 is an intracellular enzyme of 593 amino acid residues with a putative lipid-binding domain at the N-terminus. In the present study, we cloned the full-length form of the enzyme and expressed it in E. coli cells as a 6xHis-tagged protein. The majority of the expressed enzyme was found in the inclusion body of E. coli cell extracts. Upon extraction with a buffer containing urea, the recombinant enzyme was p... |
| Keywords: | Protein tyrosine phosphatase Expression Antibody production Enzyme assay Tissue expression |
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