Lectin Conjugated Gold Nanoparticle-based Colorimetric Assay for Studying the Interactions of Antibiotic with Living Cell |
| |
| Authors: | WANG Jin-e WANG Cheng-ke LIU Dian-jun WANG Zhen-xin . State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun P. R. China . Graduate School of Chinese Academy of Sciences Beijing |
| |
| Affiliation: | WANG Jin-e1,2,WANG Cheng-ke1,LIU Dian-jun1 and WANG Zhen-xin1* 1. State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P. R. China,2. Graduate School of Chinese Academy of Sciences,Beijing 100049 |
| |
| Abstract: | The interactions of antibiotic with living cells were studied by lectin conjugated gold nanoparticles(GNPs) based colorimetric assay. Because of the high affinity of lectin for saccharides, the lectin conjugated GNPs are able to employ as indicators for monitoring the antibiotic induced changes of glycosyl complexes. The interactions of a well known antibiotic, tunicamycin, with two different cell lines, HeLa and SHG-44, were selected to establish this assay. In the presence of tunicamycin, the dose- and ti... |
| |
| Keywords: | Lectin-conjugated gold nanoparticle Tunicamycin Colorimetric assay Living cell |
| 本文献已被 CNKI 等数据库收录！ |
|
