| 固相微萃取-气相色谱/质谱测定植物叶片中的挥发性物质 |
| |
| 引用本文： | 王明林,乔鲁芹,张莉,吴烈钧,田洪孝. 固相微萃取-气相色谱/质谱测定植物叶片中的挥发性物质[J]. 色谱, 2006, 24(4): 343-346 |
| |
| 作者姓名： | 王明林 乔鲁芹 张莉 吴烈钧 田洪孝 |
| |
| 作者单位： | 1.College of Food Science, Shandong Agricultural University, Taian 271018, China; 2.Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forest, Beijing 100091, China; 3.Chemical Industry Research Institute of Shandong Province, Jinan 250014, China |
| |
| 摘 要： | 采用固相微萃取（SPME)方法吸附植物叶片中的挥发性物质,然后采用气相色谱/质谱法（GC/MS）分析了挥发性物 质的成分。在45 ℃水浴温度下,采用Polyacrylate(85 μm)固相微萃取头,在广口瓶中植物叶片的上方顶空吸附60 min,然后进行GC/MS分析。结果表明,植物叶片中的挥发性物质得到了很好的分离,受山楂叶螨(Tetraychus vienneis) 危害严重的植物的完好叶片中的挥发性物质均含有顺-3-己烯-1-醇乙酸酯、顺-3-己烯-1-醇丁酸酯和α-法呢烯,且含量 较大。初步确定这些物质是对山楂叶螨具有引诱作用的主要物质,从而为利用天然生物活性物质防治山楂叶螨提供了理论 依据。
|
| 关 键 词： | 固相微萃取 挥发性物质 气相色谱 植物叶片 质谱 |
| 文章编号： | 1000-8713（2006）04-0343-04 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005-08-19 |
Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/Mass Spectrometry with Solid-Phase Microextraction |
| |
| WANG Minglin,QIAO Luqin,ZHANG Li,WU Liejun,TIAN Hongxiao. Analysis of Volatile Constituents from Leaves of Plants by Gas Chromatography/Mass Spectrometry with Solid-Phase Microextraction[J]. Chinese journal of chromatography, 2006, 24(4): 343-346 |
| |
| Authors: | WANG Minglin QIAO Luqin ZHANG Li WU Liejun TIAN Hongxiao |
| |
| Affiliation: | 1.College of Food Science, Shandong Agricultural University, Taian 271018, China; 2.Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forest, Beijing 100091, China; 3.Chemical Industry Research Institute of Shandong Province, Jinan 250014, China |
| |
| Abstract: | A method for analyzing volatile constituents from plant leaves with gas chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 degrees C in a water bath were efficiently extracted with Polyacrylate (85 microm) SPME fibers prior to perform GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead to identify biological species for preventing and treating Tetraychus vienneis. |
| |
| Keywords: | solid-phase microextraction(SPME) gas chromatography(GC) mass spectrometry(MS) volatile constituents plant leaves |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
|
点击此处可从《色谱》下载全文 |
|
