Use of near-infrared reflectance spectroscopy for shelf-life discrimination of green asparagus stored in a cool room under controlled atmosphere |
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Authors: | María-Teresa Sánchez Dolores Pérez-Marín Katherine Flores-Rojas Ana Garrido-Varo |
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Institution: | a Departamento de Bromatología y Tecnología de los Alimentos, Universidad de Cordoba, Edificio Charles Darwin, 14071 Cordoba, Spain b Departamento de Producción Animal, Universidad de Cordoba, Campus Rabanales, 14071 Cordoba, Spain |
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Abstract: | This study sought to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to classify intact green asparagus, in refrigerated storage under controlled atmosphere, by storage time and post-harvest treatments applied. A total of 468 green asparagus (Asparagus officinalis, L., cultivar UC-157) were sampled after 7, 14, 21 and 28 days of refrigerated storage (2 °C, 95% R.H.) under three controlled atmosphere (CA) treatments: air (21 kPa O2 + 0.3 kPa CO2), CA1 (5 kPa O2 + 5 kPa CO2) and CA2 (10 kPa O2 + 10 kPa CO2). Two commercially available spectrophotometers were evaluated for this purpose: a scanning monochromator (SM) of 400-2500 nm and a combination of diode array and scanning monochromator (DASM) of 350-2500 nm. Models developed using partial least squares 2-discriminant analysis (PLS2-DA) correctly classified between 81-100% of samples by post-harvest storage time, depending on the instrument used. Using similar models, the DASM instrument correctly classified 85% of samples by post-harvest treatment, compared with 72% using the SM. These results confirmed that NIR spectroscopy, coupled with the use of chemometric techniques, provides a reliable, accurate method of predicting the shelf-life of asparagus under different storage conditions and as a function of post-harvest treatment applied; the method can be readily applied at industrial level. |
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Keywords: | Green asparagus NIR spectroscopy Non-destructive analysis Shelf-life Post-harvest treatment Discriminant analysis PLS2 |
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