Synthesis of DNA-peptide conjugates by solid-phase fragment condensation |
| |
Authors: | Kubo Takanori Morikawa Masaaki Ohba Hideki Fujii Masayuki |
| |
Institution: | Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan. |
| |
Abstract: | reaction: see text] DNA-peptide conjugates were synthesized by condensing partially protected peptide fragments and oligonucleotides on a CPG support using diisocyanatoalkane as a linker. After cleavage and deprotection with aqueous ammonia, pure products were obtained by single RPHPLC purification in satisfactory yields and identified by a MALDI-TOF MS spectrometer. This method allows one to prepare DNA-peptide conjugates with any components and sequences of DNA and peptides. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|