| 同位素稀释质谱法测定蜂蜜中4种硝基呋喃代谢物 |
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| 引用本文： | 于慧梅,陈大舟,汤桦,刘娜,李蕾.同位素稀释质谱法测定蜂蜜中4种硝基呋喃代谢物[J].分析试验室,2008,27(12). |
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| 作者姓名： | 于慧梅 陈大舟 汤桦 刘娜 李蕾 |
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| 作者单位： | 1. 北京化工大学理学院,北京,100029
2. 中国计量科学研究院化学计量与分析科学研究所,北京,100013 |
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| 基金项目： | "十一五"国家科技支撑计划重点 |
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| 摘 要： | 研究了蜂蜜中4种硝基呋喃代谢物:呋喃唑酮代谢物(AOZ)、呋喃它酮代谢物(AMOZ)、呋喃妥因代谢物(AHO)、呋喃西林代谢物(SEM)的同位素稀释HPLC-MS/MS分析方法,以邻硝基苯甲醛作为衍生化试剂,AOZ-d4、AMOZ-d5、AHD-13G3、SEM·HCl-(13C,15 N2)作内标,并将超声波衍生化法应用到实际样品的分析测试当中,以乙腈-0.1%甲酸为流动相,采用梯度洗脱,15 min可将4种代谢物完全分离,再以MS/MS进行定性和定量分析.加标回收率为82%～112%;定量限(LOQ)为0.05～1 μg/kg;检出限(LOD)为0.01～0.25 μg/kg.该方法满足欧盟(EU)对进出口蜂蜜中硝基呋喃代谢物的检测要求.
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| 关 键 词： | 硝基呋喃代谢物 高效液相色谱/串联质谱 蜂蜜 同位素稀释 |
Rapid determination of four nitrofuran metabolites in honey by isotope dilution-tandem mass spectrometry |
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| YU Hui-mei,CHEN Da-zhou,TANG Hua,HU Na,Li Lei.Rapid determination of four nitrofuran metabolites in honey by isotope dilution-tandem mass spectrometry[J].Chinese Journal of Analysis Laboratory,2008,27(12). |
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| Authors: | YU Hui-mei CHEN Da-zhou TANG Hua HU Na Li Lei |
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| Institution: | YU Hui-mei1,CHEN Da-zhou2,TANG Hua2,LIU Na1 , LI Lei1(1.Department of Chemistry,College of Science,Beijing University of Chemical Technology,Beijing 100029,2.Chemical Metrology , Analytical Science Division of the National Institute of Metrology,Beijing 100013) |
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| Abstract: | A method for the determination of four nitrofuran metabolites such as 3-amino2-oxazolidinone(AOZ),5-morpholinomethyl-3-amino-2-oxazolidinone(AMOZ),1-aminohydantoin(AHD),and semicarbazide in honey has been established by isotope dilution high performance liquid chromatography-tandem mass spectrometry.Samples were derived with 2-nitrobenzaldehyde and(AOZ-d4) d4-3-amino-2-oxazolidinone,and d5-5-morpholinomethyl-3-amino-2-oxazolidinone(AMOZ-d5),13C isotope of 1-aminohydantoin(AHD-13C3),13C and 15N isotope of se... |
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| Keywords: | Nitrofuran metabolites High performance liquid chromatography-tandem mass spectrometry Honey Isotope dilution |
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