Fast microfluidic temperature control for high resolution live cell imaging |
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Authors: | Velve Casquillas Guilhem Fu Chuanhai Le Berre Mael Cramer Jeremy Meance Sebastien Plecis Adrien Baigl Damien Greffet Jean-Jacques Chen Yong Piel Matthieu Tran Phong T |
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Institution: | Institut Curie, UMR 144 CNRS, Paris, 75005, France. |
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Abstract: | One major advantage of using genetically tractable model organisms such as the fission yeast Schizosaccharomyces pombe is the ability to construct temperature-sensitive mutations in a gene. The resulting gene product or protein behaves as wildtype at permissive temperatures. At non-permissive or restrictive temperatures the protein becomes unstable and some or all of its functions are abrogated. The protein regains its function when returning to a permissive temperature. In principle, temperature-sensitive mutation enables precise temporal control of protein activity when coupled to a fast temperature controller. Current commercial temperature control devices do not have fast switching capability over a wide range of temperatures, making repeated temperature changes impossible or impractical at the cellular timescale of seconds or minutes. Microfabrication using soft-lithography is emerging as a powerful tool for cell biological research. We present here a simple disposable polydimethylsiloxane (PDMS) based microfluidic device capable of reversibly switching between 5 °C and 45 °C in less than 10 s. This device allows high-resolution live cell imaging with an oil immersion objective lens. We demonstrate the utility of this device for studying microtubule dynamics throughout the cell cycle. |
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