Application of Colloidal Gold to Fluorophotometric Determination of Human IgG |
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| Authors: | DENG Xin-yu GAO De-jiang ZUBAREV ARoman CHEN Yan-hua YU Ai-min ZHANG Han-qi WANG Xing-hua CHEN Ying |
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| Institution: | [1]College of Chemistry, Jilin University, Changchun 130012, P. R. China [2]Jilin Province Center of Environmental Monitoring, Changchun 130011, P R. China |
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| Abstract: | A method for the determination of human immunoglobulin G(IgG) based on a colloidal gold label by fluorospectrophotometry was developed. The sandwich immunoreaction among goat-anti-human IgG, human IgG and goat-anti-human IgG labeled with colloidal gold nanoparticles was applied in this experiment. First, a sandwich immunocomplex was formed on the surface of 96 well clear polystyrene high bind stripwellTM microplate. After the formation of the sandwich immunocomplex, a solution was added to dissociate the im... |
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| Keywords: | Human IgG Colloidal gold Fluorospectrophotometry |
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