Biosynthesis,purification and structure-property relationships of new cyanobacterial exopolysaccharides |
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| Affiliation: | 1. i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal;2. IBMC - Instituto de Biologia Celular e Molecular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal;3. ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal;4. IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal;5. FMUP - Faculty of Medicine, Department of Pathology, University of Porto, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;6. DAGRI - Department of Agriculture, Food, Environment and Forestry, University of Florence, Via Maragliano 77, 50144 Firenze, Italy;7. INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal;8. FEUP - Faculdade de Engenharia, Departamento de Engenharia Metalúrgica e Materiais, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal;9. FCUP - Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007 Porto, Portugal |
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| Abstract: | Exopolysaccharides are considered as omnipresent and abundant bioproducts. However, the diversity of cyanobacteria offers a great opportunity for discovering new bioproducts as they are not properly explored. The current work targeted to characterize an indigenous cyanobacterial strain CCC-746 isolated from Bishnupur, India for production of exo-polysaccharides. The molecular and microscopic observation stated that the isolated cyanobacteria is Anabaena sp. The analysis of biomass composition showed carbohydrates, protein, lipid, ash and moisture content are 32.10%, 36.56%, 24.13%, 5.89% and 1.32% (w/w) respectively. The maximum production of EPS was 340.70 mg/L, when grown in 10 mM CaCl2 supplemented BG11 medium. The capsular polysaccharide and released exo-polysaccharide were purified by gel filtration chromatography, and molecular weight was found as 12.52 and 9.2 kDa, respectively. The major component of both exo-polysaccharides was carbohydrate with glucose, xylose and glucuronic acid as major monosaccharides. Capsular polysaccharide and released exo-polysaccharide were composed of [→4)-Glcp-(1→] backbone. Both exo-polysaccharide were found negative in charge and showed remarkable antioxidant and scavenging activities. Capsular polysaccharide and released exo-polysaccharide solubilized in aqueous solution exhibited the behaviour of pseudo-plastic fluids. |
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| Keywords: | Capsular polysaccharide Released exo-polysaccharides Structure-property relationship Pseudoplastic fluid |
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