Protein A-conjugated luminescent gold nanodots as a label-free assay for immunoglobulin G in plasma |
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Authors: | Shiang Yen-Chun Lin Che-An Huang Chih-Ching Chang Huan-Tsung |
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Institution: | Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan. |
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Abstract: | We have employed protein A-modified gold nanodots (PA-Au NDs) as a luminescence sensor for the detection of human immunoglobulin G (hIgG) in homogeneous solutions. The luminescent PA-Au NDs were prepared simply by mixing protein A with the luminescent Au NDs (average diameter: ca. 1.8 nm). The specific interactions that occur between protein A and hIgG allowed us to use the PA-Au NDs to detect hIgG selectively. Under optimal conditions 10 nM PA-Au NDs (two protein A molecules per Au ND), 5.0 mM phosphate buffer solution, pH 7.4], the PA-Au ND probe detected hIgG with high sensitivity (limit of detection = 10 nM) and remarkable selectivity (>50-fold) over other proteins. In an assay that took advantage of the competition between protein G and the PA-Au NDs for IgG, we detected protein G at concentrations as low as 85 nM. This PA-Au ND probe allowed determination of the hIgG concentration in plasma samples without any need for sample pretreatment. Our results exhibited a good linear correlation (R(2)=0.97) with those obtained using an enzyme-linked immunosorbent assay. Our simple, sensitive, and selective approach appears to hold practical potential for use in the clinical diagnosis of immune diseases associated with changes in hIgG levels. |
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