Development and validation of a highly sensitive LC-MS/MS method for simultaneous quantitation of ethionamide and ethionamide sulfoxide in human plasma: application to a human pharmacokinetic study |
| |
Authors: | Deshpande Abhijeet Y Gurav Sandip Punde Ravindra Zambre Vishal Kulkarni Rahul Pandey Sarvesh Mungantiwar Ashish Mullangi Ramesh |
| |
Affiliation: | Bioanalytical Department, Bioequivalence Centre, Macleods Pharmaceutical Ltd, Mahakali Caves Road, Shanti Nagar, Andheri (E), Mumbai-400093, India. |
| |
Abstract: | A highly sensitive and specific LC‐MS/MS method has been developed for simultaneous quantification of ethionamide and ethionamide sulfoxide in human plasma (300 µL) using prothionamide as an internal standard (IS). Solid‐phase extraction was used to extract ethionamide, ethionamide sulfoxide and IS from human plasma. The chromatographic separation of ethionamide, ethionamide sulfoxide and IS was achieved with a mobile phase consisting of 0.1% acetic acid : acetonitrile (20:80, v/v) at a flow rate of 0.50 mL/min on a Peerless Basic C18 column. The total run time was 3.5 min and the elution of ethionamide, ethionamide sulfoxide and IS occurred at 2.50, 2.18 and 2.68 min, respectively. A linear response function was established for the range of concentrations 25.7–6120 ng/mL (r > 0.998) for ethionamide and 50.5–3030 ng/mL (r > 0.998) for ethionamide sulfoxide. The intra‐ and inter‐day precision values for ethionamide and ethionamide sulfoxide met the acceptance as per FDA guidelines. Ethionamide and ethionamide sulfoxide were stable in battery of stability studies, viz. bench‐top, autosampler and freeze–thaw cycles. The developed assay was applied to a pharmacokinetic study in humans. Copyright © 2011 John Wiley & Sons, Ltd. |
| |
Keywords: | ethionamide ethionamide sulfoxide LC‐MS/MS method validation human plasma pharmacokinetics |
本文献已被 PubMed 等数据库收录! |
|