Synthesis of RNA using 2'-O-DTM protection |
| |
Authors: | Semenyuk Andrey Földesi Andras Johansson Tommy Estmer-Nilsson Camilla Blomgren Peter Brännvall Mathias Kirsebom Leif A Kwiatkowski Marek |
| |
Affiliation: | Department of Genetics and Pathology, Uppsala University, Sweden. |
| |
Abstract: | tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2'-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2'-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5'-O-DMTr function can be used for high-throughput cartridge RNA purification. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|