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Fluorescence monitoring of microchip capillary electrophoresis separation with monolithically integrated waveguides
Authors:Dongre Chaitanya  Dekker Ronald  Hoekstra Hugo J W M  Pollnau Markus  Martinez-Vazquez Rebeca  Osellame Roberto  Cerullo Giulio  Ramponi Roberta  van Weeghel Rob  Besselink Geert A J  van den Vlekkert Hans H
Affiliation:Integrated Optical MicroSystems, MESA Institute for Nanotechnology, University of Twente, AE Enschede, The Netherlands. C.Dongre@ewi.utwente.nl
Abstract:Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12 microm, enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution.
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