| Quantitation of PCR Products by Capillary Electrophoresis in a Single Run |
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| 引用本文： | Fu Tian HAN, Bing Cheng LIN(Dalian Institute of Chendcal Physics,Chinese Academy of Sciences,Dalian 116023). Quantitation of PCR Products by Capillary Electrophoresis in a Single Run[J]. 中国化学快报, 1998, 0(6) |
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| 作者姓名： | Fu Tian HAN Bing Cheng LIN(Dalian Institute of Chendcal Physics Chinese Academy of Sciences Dalian 116023) |
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| 作者单位： | Dalian Institute of Chendcal Physics，Chinese Academy of Sciences，Dalian 116023 |
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| 摘 要： | IntroductionItisnecessarytoevaluatetheamountofDNAfragmentinsuchcasesasgeneexpressioninvestigati0n.HybridizationwithradioactivitytaggedoligonucleotidesfollowedbyphotograPhyanddensitometricscanisoftenemployedtomeettheneedsofsuchrequirementS,whichislaborintensive,timec0nsumingandunautomated-CaPillaryelectrophoresis(CE)tendst0beastrongtoolintheanalysis0fDNAfragmentSwiththeadvantagesofhighsensitivity,highres0luti0nandautomation.QuantitativeanalysisofDNAfragmentshasbeendonebysomeauthorsusingC…
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Quantitation of PCR Products by Capillary Electrophoresis in a Single Run |
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| Fu Tian HAN, Bing Cheng LIN. Quantitation of PCR Products by Capillary Electrophoresis in a Single Run[J]. Chinese Chemical Letters, 1998, 0(6) |
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| Authors: | Fu Tian HAN Bing Cheng LIN |
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| Abstract: | A simple method was developed to quantify DNA fragments such as PCR (polymerasechain reaction) products by capillary electrophoresis. Restraint fragments with different lengthswere employed as internal standards in the study, which makes it possible for the evaluation of thequantity of PCR product in a single run. |
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| Keywords: | capillary electrophoresis DNA quantitation PCR products |
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