气相色谱法分析尿液样品中的阿特拉津及其代谢物

建立了尿液样品中阿特拉津(ATZ)及其代谢物脱乙基阿特拉津(DEA)、脱异丙基阿特拉津(DIA)、脱乙基脱异丙基阿特拉津(DEDIA)的气相色谱分析方法。样品经乙酸乙酯萃取、硫酸钠脱水、弗罗里硅土净化、浓缩后用气相色谱-电子俘获检测器分析。对样品萃取时的pH值等条件进行了优化,获得了较好的回收率。方法的检出限分别为DEDIA 0.0025 mg/L,DEA、DIA、ATZ 0.005 mg/L。4种化合物在进样量为0.2~8 ng时与其峰面积呈良好的线性关系。利用该方法对阿特拉津生产厂工人的尿液样品进行了分析，尿液中4种化合物的质量浓度为：DEDIA 0.003～0.301 mg/L,DEA 0.005～0.011 mg/L,DIA 0.006～0.276 mg/L,ATZ 0.005～0.012 mg/L。

Determination of Atrazine and Its Metabolites in Human Urines Using Gas Chromatography

Atrazine(ATZ) was widely used to control broadleaf weeds.Numerous animal experiments have proved that atrazine is a suspicious endocrine disruptor.Thus,further development of the ability to estimate low-dose human exposure to atrazine is requested in epidemiologic studies to correlate the toxicological effects associated with the concentrations of ATZ and its metabolites in human body.A method for detecting ATZ and its metabolites(deethylatrazine(DEA),deisopropylatrazine(DIA),deethyldeisopropylatrazine(DEDIA)) in human urines using gas chromatography was established.A urine sample was extracted by ethyl acetate,and purified using a Florisil column.Final concentrated extract was analyzed by a gas chromatograph-electron capture detector.The conditions of this method were optimized.The limits of detection were 0.002 5 mg/L for DEDIA,0.005 mg/L for DEA,DIA and ATZ.The linear ranges were from 0.2 to 8 ng for all analytes.The atrazine concentrations in urine samples of the workers collected from an atrazine plant were determined by this method.The concentration ranges were 0.003-0.301 mg/L for DEDIA,0.005-0.011 mg/L for DEA,0.006-0.276 mg/L for DIA,and 0.005-0.012 mg/L for ATZ.