Coupling of chromatographic ion-exchange reaction with change-partner reaction. Presumed mechanism on the ion-ion interaction of inorganic ions in Sephadex G-15 columns |
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Authors: | Okada T K Nakabayashi Y Jisaki F |
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Affiliation: | Department of Biochemistry, Kanazawa Medical University, Uchinada, Ishikawa, Japan. tk-okada@kanazawa-med.ac.jp |
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Abstract: | A sample solution containing sodium or potassium dihydrogenphosphate or disodium or dipotassium hydrogenphosphate was eluted from a Sephadex G-15 column with either sodium or potassium chloride solution in various sample-eluent systems, and every one of the four kinds of ions employed was determined in the eluate. Then, the elution profiles showed the phenomenon that a negative peak of chloride ion coexisted in the fractions of a positive peak of phosphate ion, in all sample-eluent systems employed. This phenomenon was assumed to occur by the coupled reaction of change-partner and ion-exchange reactions including ion exclusion. |
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