The Neovessel Occlusion Efficacy of 151-Hydroxypurpurin-7-Lactone Dimethyl Ester Induced with Photodynamic Therapy |
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| Authors: | Siang Hui Lim Patrycja Nowak-Sliwinska Fadzly Adzhar Kamarulzaman Hubert Van Den Bergh Georges Wagnières Hong Boon Lee |
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| Affiliation: | 1. Cancer Research Initiatives Foundation (CARIF), Sime Darby Medical Centre, Subang Jaya, Selangor, Malaysia;2. Institute of Bio-Engineering, Medical Photonics Group, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland |
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| Abstract: | In this study, the photodynamic therapy (PDT) induced efficacy of a semi-synthesized analogue 151-hydroxypurpurin-7-lactone dimethyl ester or G2, in terms of chick chorioallantoic membrane blood vessel occlusion was evaluated in reference to verteporfin. Early formulation studies showed that G2 prepared in a system of cremophor EL 2.5% and ethanol 2.5% in saline was biocompatible up to 20 μL volume of injection. Following injection, G2 accumulation peaked within the first minute and its extravasation from intra- to extra-vascular occurred somewhat slower as compared with verteporfin. In the PDT study, closure of capillaries and small neovessels was observed with 4 μg per embryo of G2 and a light dose of 20 J cm−2 at a fluence rate of 40 mW cm−2 filtered at 400–440 nm—a result that may be considered optimum for the treatment of age-related macular degeneration (AMD). Also, partial occlusion of the large vessels was observed using the same dose of G2 and light—an effect which is desirable for cancer treatment. From this study, we conclude that G2 has the potential to be developed as a therapeutic agent for photodynamic treatment for AMD and cancer. |
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