Magnetic separation as a new method for the extraction of small molecules from biological fluids of humans |
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Authors: | I I Sukhanova M A Dikunets E D Viryus G M Rodchenkov |
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Institution: | 1.FSUE Anti-Doping Center,Ministry of Sport, Tourism, and Youth Policy of the Russian Federation,Moscow,Russia |
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Abstract: | A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation
using ferromagnetic microparticles with the surface modified with C18 groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome
proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry.
Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative
effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase
extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of
pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25–5
ng/mL. |
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