Fluorescence Imaging of Stem Cells,Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots |
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Authors: | M Vibin R Vinayakan Annie John V Raji C S Rejiya Annie Abraham |
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Institution: | (1) Department of Biochemistry, University of Kerala, Thiruvananthapuram, 695581 Kerala, India;(2) Photosciences and Photonics, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019 Kerala, India;(3) Sree Chitra Tirunal Institute of Medical Sciences and Technology, Biomedical Technology Wing, Thiruvananthapuram, 695012 Kerala, India; |
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Abstract: | Trioctylphosphine oxide capped cadmium selenide quantum dots, synthesized in organic media were rendered water soluble by
silica overcoating. Silanisation was done by a simple reverse microemulsion method using aminopropyl silane as the silica
precursor. Further, the strong photoluminescence of the silica-coated CdSe quantum dots has been utilized to visualize rabbit
adipose tissue-derived mesenchymal stem cells (RADMSCs) and Daltons lymphoma ascites (DLA) cancerous cells in vitro. Subsequently
the in vivo fluorescence behaviours of QDs in the tissues were also demonstrated by intravenous administration of the QDs
in Swiss albino mice. The fluorescence microscopic images in the stem cells, cancer cells and semi-thin sections of mice organs
proved the strong luminescence property of silica-coated quantum dots under biological systems. These results establish silica-coated
CdSe QDs as extremely useful tools for molecular imaging and cell tracking to study the cell division and metastasis of cancer
and other diseases. |
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