Laser-induced fluorescence and UV detection of derivatized aldehydes in air samples using capillary electrophoresis |
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Authors: | Pereira Elisabete Alves Carrilho Emanuel Tavare Marina F M |
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Institution: | Departamento de Química Fundamental, Instituto de Química, Universidade de S?o Paulo, Av. Prof. Lineu Prestes 748, 05508-900 S?o Paulo, SP, Brazil. |
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Abstract: | In this work, two capillary zone electrophoresis methodologies using UV absorption detection (214 nm) and laser-induced fluorescence detection (He/Cd laser, 325 nm excitation, 520 nm emission) of selected aldehydes (formaldehyde, acetaldehyde, propionaldehyde and acrolein) derivatized with dansylhydrazine (DNSH, 5-dimethylaminonaphthalene-1-sulfohydrazide) were proposed and validated. The aldehydes react with DNSH to form negatively charged molecules in methanolic medium. In both methodologies, nine DNSH-derivatives, including isomers of acetaldehyde, propionaldehyde and acrolein and two impurities were baseline separated in 20 mmol l(-1) phosphate buffer at pH 7.02, in less than 9 min. The limits of detection for the UV and LIF methodologies ranged from 1.1-9.5 microg l(-1) and 0.29-5.3 microg l(-1), respectively. The applicability of both methodologies to contemplate real samples was confirmed in the analysis of aldehyde-DNSH derivatives in indoor and outdoor air samples. |
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