Direct fluorescence detection of non-steroidal anti-inflammatory agents separated by TLC with 9-isothiocyanatoacridine derivatives |
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| Authors: | C Sârbu C M?ru?oiu M Vlassa |
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| Institution: | (1) Faculty of Mechanical Engineering, Polytechnic Institute, 3400 Cluj-Napoca, Romania;(2) Institute of Chemistry, 3400 Cluj-Napoca, Romania |
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| Abstract: | Summary Non-steroidal anti-inflammatory agents were separated by thin-layer chromatography, utilizing precoated plates with silica
gel R as the stationary phase and chloroform:methanol:25% ammonia (80∶15∶5), as the mobile phase.
The spots were visualized by spraying with 0.1% solution of 9-isothiocyantoacridine derivatives in methylene chloride or benzene
and irradiating with UV at 254 and 366nm. The visual detection limit of a spot was 0.1μg. |
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| Keywords: | Thin-layer chromatography Anti-inflammatory agents 9-Isothiocyanatoacridine derivatives Fluorescence detection |
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