| 荧光介孔二氧化硅纳米粒子的合成及药物运输 |
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| 引用本文： | 陈敏敏,耿浩然,胡金霞,张琼,Godfred Amfo Agyekum,张卓琦,曹希传. 荧光介孔二氧化硅纳米粒子的合成及药物运输[J]. 无机化学学报, 2019, 35(11): 2125-2135 |
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| 作者姓名： | 陈敏敏 耿浩然 胡金霞 张琼 Godfred Amfo Agyekum 张卓琦 曹希传 |
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| 作者单位： | 中国矿业大学材料科学与工程学院, 徐州 221000;中国矿业大学化工学院, 徐州 221000,中国矿业大学材料科学与工程学院, 徐州 221000,中国矿业大学材料科学与工程学院, 徐州 221000;中国矿业大学化工学院, 徐州 221000,徐州医科大学附属医院心内科, 徐州 221000,徐州医科大学附属医院心内科, 徐州 221000,徐州医科大学附属医院心内科, 徐州 221000,中国矿业大学材料科学与工程学院, 徐州 221000 |
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| 基金项目： | 中央高校基本科研业务费专项资金（No.2018BSCXB11）和江苏省研究生科研与实践创新计划项目（No.KYCX18_1937）资助 |
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| 摘 要： | 合成了荧光介孔二氧化硅纳米粒子（MSNs-FITC），并研究了其在持续药物释放和生物示踪成像方面的应用。首先，采用一步法合成出MSNs-FITC，结合SEM、TEM、FT-IR、XRD和氮气吸附脱附等表征技术进行表征。其次，将抗癌药物阿霉素（DOX）负载到MSNs-FITC中。载药粒子的药物释放行为具有明显的pH依赖性，酸性环境加速释放速率。同时，体外细胞毒性测试表明MSNs-FITC具有良好的生物相容性。激光共聚焦扫描显微镜（CLSM）图像表明，MSNs-FITC可以进入细胞并具有剂量依赖性，流式细胞术分析（FCM）进一步证明了这一结果。
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| 关 键 词： | 介孔二氧化硅纳米载体 药物运输 细胞毒性 生物成像 |
| 收稿时间： | 2019-08-13 |
| 修稿时间： | 2019-10-04 |
Synthesis of Fluorescent Mesoporous Silica Nanoparticles and Application for Intracellular Drug Delivery |
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| CHEN Min-Min,GENG Hao-Ran,HU Jin-Xi,ZHANG Qiong,Godfred Amfo Agyekum,ZHANG Zhuo-Qi and CAO Xi-Chuan. Synthesis of Fluorescent Mesoporous Silica Nanoparticles and Application for Intracellular Drug Delivery[J]. Chinese Journal of Inorganic Chemistry, 2019, 35(11): 2125-2135 |
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| Authors: | CHEN Min-Min GENG Hao-Ran HU Jin-Xi ZHANG Qiong Godfred Amfo Agyekum ZHANG Zhuo-Qi CAO Xi-Chuan |
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| Affiliation: | School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China;School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China,School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China,School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China;School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China,Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China,Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China,Department of Cardiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China and School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221000, China |
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| Abstract: | Here, we reported the synthesis and FITC-functionalized of mesoporous silica nanoparticles (MSNs) for tracing bioimaging and sustained drug release. Firstly, the MSNs-FITC were synthesized through one-pot method and characterized by SEM, TEM, FT-IR, XRD and the N2 adsorption-desorption isotherm respectively. Then a classic anticancer drug doxorubicin (DOX) was loaded into the MSNs-FITC. The DOX-loaded nanocarriers exhibited obviously pH dependent release behavior with accelerated release rate in acidic environment. Meanwhile, the in vitro cytotoxicity evaluation exhibited that the nude MSNs-FITC were highly biocompatible. Furthermore, the confocal laser scanning microscope (CLSM) images indicated that MSNs-FITC could penetrate into living cells effectively and dose-depended internalization of MSNs-FITC were further investigated by flow cytometry analysis (FCM). |
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| Keywords: | mesoporous silica nanocarriers drug delivery cytotoxicity bioimaging |
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