| 一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用 |
| |
| 引用本文： | 王成,杨曼,邓祥义,何明礼. 一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用[J]. 无机化学学报, 2020, 36(4): 762-768 |
| |
| 作者姓名： | 王成 杨曼 邓祥义 何明礼 |
| |
| 作者单位： | 湖北理工学院环境科学与工程学院, 黄石 435003,湖北理工学院环境科学与工程学院, 黄石 435003,湖北理工学院化学与化工学院, 黄石 435003,武汉理工大学安全科学与应急管理学院, 武汉 430070 |
| |
| 基金项目： | 国家自然科学基金（No.51372077）资助项目。 |
| |
| 摘 要： | 合成和表征了一种用于检测HSO3^-的苯并咪唑类荧光增强型探针(FP1)。结果表明:FP1对HSO3^-表现出了明显的荧光关-开响应,具有高的选择性和灵敏度,且抗干扰能力强,响应时间短(2 min)。FP1的荧光强度与HSO3^-的浓度在1.2~8.1μmol·L^-1的范围内呈线性关系,其对HSO3^-的检出限为0.14μmol·L^-1。此外,FP1能用于实际样品中HSO3^-的检测,回收率良好,它还可应用于HeLa活细胞中对HSO3&-进行荧光成像。
|
| 关 键 词： | 亚硫酸氢根 荧光探针 苯并咪唑 检测 荧光成像 |
| 收稿时间： | 2019-09-12 |
| 修稿时间： | 2019-12-23 |
A Fluorescence-Enhanced Probe Based on Benzimidazole for Bisulfite and Its Practical Application |
| |
| WANG Cheng,YANG Man,DENG Xiang-Yi and HE Ming-Li. A Fluorescence-Enhanced Probe Based on Benzimidazole for Bisulfite and Its Practical Application[J]. Chinese Journal of Inorganic Chemistry, 2020, 36(4): 762-768 |
| |
| Authors: | WANG Cheng YANG Man DENG Xiang-Yi HE Ming-Li |
| |
| Affiliation: | School of Environmental Science and Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China,School of Environmental Science and Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China,College of Chemistry and Chemical Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China and School of Safety Science and Emergency Management, Wuhan University of Technology, Wuhan 430070, China |
| |
| Abstract: | A benzimidazole-based fluorescent turn-on probe(FP1)for the detection of HSO3-has been synthesized and characterized.The results showed that FP1 exhibited a clear off-on fluorescence response toward HSO3-,and it was highly selective and sensitive to HSO3-with strong anti-interference and a short response time(2 min).The fluorescence intensity of FP1 varied linearly with the concentration of HSO3-in the range of 1.2~8.1μmol·L^-1,and the detection limit for HSO3-was estimated to be 0.14μmol·L^-1.In addition,FP1 can be used to detect HSO3-in real samples with good recovery,and it was also applicable for fluorescence imaging of HSO3-in living HeLa cells. |
| |
| Keywords: | bissulfite fluorescent probe benzimidazole determination fluorescent imaging |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《无机化学学报》浏览原始摘要信息 |
|
点击此处可从《无机化学学报》下载全文 |
|
