| 高效液相色谱法测定生物催化法生产中的己二酸 |
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| 引用本文： | 王净,田晶,于玲,赵晓明,许国旺. 高效液相色谱法测定生物催化法生产中的己二酸[J]. 分析科学学报, 2006, 22(5): 530-532 |
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| 作者姓名： | 王净 田晶 于玲 赵晓明 许国旺 |
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| 作者单位： | 中国科学院大连化学物理研究所国家色谱中心,辽宁,大连,116011;大连轻工业学院分析中心,辽宁,大连,116034;大连轻工业学院分析中心,辽宁,大连,116034;中国科学院大连化学物理研究所国家色谱中心,辽宁,大连,116011 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);中国科学院知识创新工程项目 |
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| 摘 要： | 己二酸是一种重要有机化合物,具有强极性和离子性的特点,由于在210 nm以上有弱的紫外吸收,故用气相色谱(GC)或高效液相色谱(HPLC)直接分离测定均有一定困难。为此,本文从研究乙酸缓冲溶液中己二酸-铜络合物的紫外吸收光谱出发,建立了高效液相色谱紫外检测己二酸的方法。通过实验确定了最优色谱条件。本法测定己二酸的线性相关系数为0.998,回收率为97.5%,检出限为0.32 mg/mL。此方法可用于监测生物催化法生产的己二酸,优化生产条件。
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| 关 键 词： | 高效液相色谱 己二酸 二元羧酸-铜络合物 生物催化 |
| 文章编号： | 1006-6144(2006)05-0530-03 |
| 收稿时间： | 2005-08-16 |
| 修稿时间： | 2005-11-16 |
Determination of Adipic Acid in the Process of Biocatalysis by High Performance Liquid Chromatography |
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| WANG Jing,TIAN Jing,YU Ling,ZHAO Xiao-ming,XU Guo-wang. Determination of Adipic Acid in the Process of Biocatalysis by High Performance Liquid Chromatography[J]. Journal of Analytical Science, 2006, 22(5): 530-532 |
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| Authors: | WANG Jing TIAN Jing YU Ling ZHAO Xiao-ming XU Guo-wang |
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| Affiliation: | 1. National Chromatography Research CAS, 2. Dalian Institute of and Analysis Center, Dalian Institute of Chemical Physics, Dalian, Liaoning 116023 Light Industry ,Dalian,Liaoning 116034 |
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| Abstract: | Biocatalysis is a new way used in chemical field.Biological processes are commonly perceived as being less harmful to environment than chemical manufacturing processes.To analyze adipic-acid produced by biocatalysis,adipic acid-copper complex was analyzed by high performance liquid chromatography with UV-detector.The effects of mobile phase such as the concentration of methyl alcohol,copper ions and buffer as well as pH values were discussed.Guidelines were given for optimization of the separation.The separation was carried out on a C18 column with the flow rate of mobile phase of 0.6 mL/min and the detection wavelength of 254 nm. |
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| Keywords: | High performance liquid chromatography Adipic acid Copper Biocatalysis |
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