| 液相色谱-质谱联用分离、鉴定硫代葡萄糖苷 |
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| 引用本文： | 袁丽凤,郭伟强,王志刚. 液相色谱-质谱联用分离、鉴定硫代葡萄糖苷[J]. 浙江大学学报(理学版), 2004, 31(2): 180-183 |
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| 作者姓名： | 袁丽凤 郭伟强 王志刚 |
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| 作者单位： | 浙江大学,化学系,浙江,杭州,310028 |
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| 摘 要： | 从油菜籽中提取硫苷,经弱阴离子交换剂DEAE Sephadex A-25做预处理,得到硫苷提取液.经反相离子对色谱确定分离条件,采用液相色谱-电喷雾接口-质谱联用,再经紫外扫描进一步确证,同时分离鉴定了油菜籽中7种硫苷.
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| 关 键 词： | 硫苷 反相离子对色谱 液相色谱-质谱联用 |
| 文章编号： | 1008-9497(2004)02-180-04 |
| 修稿时间： | 2003-09-08 |
Determination of glucosinolates by liquid chromatography-mass spectrometry |
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| YUAN Li-feng,GUO Wei-qiang,WANG Zhi-gang. Determination of glucosinolates by liquid chromatography-mass spectrometry[J]. Journal of Zhejiang University(Sciences Edition), 2004, 31(2): 180-183 |
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| Authors: | YUAN Li-feng GUO Wei-qiang WANG Zhi-gang |
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| Abstract: | A reliable method to separate and identify glucosinolates in rapeseed was developed by using reversed-phase ion-pair HPLC with confirmation by electrospray mass spectrometry and ultraviolet spectroscopy. The extracting solution of glucosinolates was first purified by a column packed with DEAE-sephadex A-25, and then was injected into the chromatographic system. Confirmation of identity was accomplished using both negative ion electrospray mass spectrometry and ultraviolet spectroscopy. Seven species of glucosinolates were determined by using this method. |
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| Keywords: | DEAE Sephadex A-25 |
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