Split injection: A simple introduction of subnanoliter sample volumes for chip electrophoresis |
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Authors: | A Gáspár PI Koczka H Carmona FA Gomez |
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Institution: | aDepartment of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, Hungary;bDepartment of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA |
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Abstract: | The ability to accurately inject small volumes of sample into microfluidic channels is of great importance in electrophoretic separations. While electrokinetic injection of nanoliter scale volumes is commonly utilized in microchip capillary electrophoresis (MCE), mobility and matrix bias makes quantitation difficult. Herein, we describe a new injection method based on the simple patterning of the crossing of channels that does not require sophisticated instrumentation. The sample volume injected into the separation channel is dependent on the ratio of the widths of the crossing channels. This injection method is capable of introducing, into a separation channel, multiple plugs of sample on a large scale. This injection technique is tested for zone electrophoresis in native and surface modified poly(dimethylsiloxane) (PDMS) chips. |
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Keywords: | Chip Poly(dimethylsiloxane) Pressure injection Chip electrophoresis |
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