| A STRATEGY FOR ISOLATING DIFFERENTIATIONINDUCING COMPLEMENTARY DNAs FROM HUMAN ESOPHAGEAL CANCER CELL LINE TREATED WITH RETINOIC ACID |
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| 作者姓名： | 冯骆 王秀琴 傳明 王志华 田园 蔡岩 吴旻 |
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| 作者单位： | National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC，National Laboratory of Molecular Oncology，Department of Cell Biology，Cancer Institute，Chinese Academy of Medical Sciences，Beijing 100021，PRC |
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| 基金项目： | Project supported by 863 High-Tech Foundation & World Lab. |
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| 摘 要： | Treatment of the human esophageal cancer cell line EC8712 with retinoic acid (RA) stopped the cell growth significantly and gave rise to terminal differentiation of the cells characterized by increased expression of involucrin gene. Two cDNA libraries were constructed from the parental and RA-treated cells respectively. Repeated subtractive hybridization of single-stranded plasmid DNA prepared from pooled colonies of cDNA library of the parental cells with cDNA probe generated from the RA-treated cells exhausted sequences common to both libraries of the cell. The unhybridized cDNA probe represented, therefore, the genes activated after RA-treatment. By using these enriched cDNAs as probe to screen the cDNA library constructed from the RA-treated cells thirty-nine positive colonies were obtained, of which two were specifically due to RA-induction. One of these two cDNA clones, designated as pRA538, has undergone further analysis and shown differentiation-inducing effect on parental cancer cells. A novel
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A STRATEGY FOR ISOLATING DIFFERENTIATIONINDUCING COMPLEMENTARY DNAs FROM HUMAN ESOPHAGEAL CANCER CELL LINE TREATED WITH RETINOIC ACID |
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| FENG LUO WANG XIU-QIN FU MING WANG ZHI-HUA TIAN YUAN CAI YAN AND WU MIN.A STRATEGY FOR ISOLATING DIFFERENTIATIONINDUCING COMPLEMENTARY DNAs FROM HUMAN ESOPHAGEAL CANCER CELL LINE TREATED WITH RETINOIC ACID[J].Science in China(Chemistry),1992(4). |
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| Authors: | FENG LUO WANG XIU-QIN FU MING WANG ZHI-HUA TIAN YUAN CAI YAN AND WU MIN |
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| Abstract: | Treatment of the human esophageal cancer cell line EC8712 with retinoic acid (RA) stopped the cell growth significantly and gave rise to terminal differentiation of the cells characterized by increased expression of involucrin gene. Two cDNA libraries were constructed from the parental and RA-treated cells respectively. Repeated subtractive hybridization of single-stranded plasmid DNA prepared from pooled colonies of cDNA library of the parental cells with cDNA probe generated from the RA-treated cells exhausted sequences common to both libraries of the cell. The unhybridized cDNA probe represented, therefore, the genes activated after RA-treatment. By using these enriched cDNAs as probe to screen the cDNA library constructed from the RA-treated cells thirty-nine positive colonies were obtained, of which two were specifically due to RA-induction. One of these two cDNA clones, designated as pRA538, has undergone further analysis and shown differentiation-inducing effect on parental cancer cells. A novel strategy for cloning genes involved in terminal differentiation of cancer cells is developed. |
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| Keywords: | differentiation gene tumor suppressor gene retinoic acid esophageal cancer |
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