Micromechanical measurement of AChBP binding for label-free drug discovery |
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Authors: | Buchapudi Koutilya Xu Xiaohe Ataian Yeganeh Ji Hai-Feng Schulte Marvin |
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Institution: | Department of Chemistry, Drexel University, Philadelphia, PA 19104, USA. |
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Abstract: | A potential binding assay based on binding-driven micromechanical motion is described. Acetylcholine binding protein (AChBP) was used to modify a microcantilever. The modified microcantilever was found to bend on application of the naturally occurring agonist (acetylcholine) or the antagonist (nicotine and d-tubocurarine). Control experiments show that microcantilevers modified without AChBP do not respond to acetylcholine, nicotine, and d-tubocurarine. K(d) values obtained for acetylcholine, nicotine, and d-tubocurarine are similar to those obtained from radio-ligand binding assays. These results suggest that the microcantilever system has potential for use in label free, drug screening applications. |
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