| 藏红T与六偏磷酸钠的相互作用及在食品分析中的应用 |
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| 引用本文： | 乐敏,李原芳. 藏红T与六偏磷酸钠的相互作用及在食品分析中的应用[J]. 应用化学, 2011, 28(9): 1087-1092. DOI: 10.3724/SP.J.1095.2011.00627 |
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| 作者姓名： | 乐敏 李原芳 |
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| 作者单位： | (发光与实时分析教育部重点实验室,西南大学化学化工学院 重庆400715) |
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| 基金项目： | 国家自然科学基金资助项目(90813019) |
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| 摘 要： | 对阳离子荧光染料藏红T（ST）在食品添加剂六偏磷酸钠（SHMP）存在时的溶液状态的荧光光谱及吸收光谱进行了研究。 结果发现，在醋酸-醋酸钠缓冲条件下，SHMP使ST溶液发生自聚集，同时因静电作用二者形成杂化聚集体，导致ST的荧光强度和吸光度降低且在476 nm处出现新的吸收峰,但ST的荧光寿命没有发生明显的变化，表明SHMP静态猝灭ST的荧光。 根据SHMP存在时ST吸光度的变化，建立了一种检测食品添加剂六偏磷酸钠的新方法，线性范围为1.0×10-6~1.1×10-5 mol/L，检测限为3.4×10-7 mol/L。 方法成功应用于饮料中六偏磷酸钠的测定，相对标准偏差小于4.6%，回收率在95.0%~104.0%之间。
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| 关 键 词： | 藏红T 六偏磷酸钠 荧光光谱 吸收光谱 |
| 收稿时间： | 2010-10-20 |
| 修稿时间： | 2010-12-10 |
The Interaction of Safranine T with Sodium Hexametaphosphate and Its Application in Food Analysis |
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| LE Min,LI Yuanfang. The Interaction of Safranine T with Sodium Hexametaphosphate and Its Application in Food Analysis[J]. Chinese Journal of Applied Chemistry, 2011, 28(9): 1087-1092. DOI: 10.3724/SP.J.1095.2011.00627 |
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| Authors: | LE Min LI Yuanfang |
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| Affiliation: | (Education Ministry Key Laboratory on Luminescence and Real-time Analysis,;College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715) |
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| Abstract: | The visible spectra and fluorescent spectra of safranine T(ST) solutions with the presence of sodium hexametaphosphat(SHMP) were investigated.The results showed that ST tends to aggregate when SHMP was presented and a heteropolymer appeared by electrostatic attraction between ST and SHMP in the HAc-NaAc buffer solution,which results in a new peak appeared in 476 nm in visible absorption spectra and the intensity of the absorbance and the fluorescence decreased.Meanwhile,the fluorescent lifetime of the ST wa... |
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| Keywords: | safranine T sodium hexametaphosphat fluorescence spectra absorbance spectra |
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