姜黄素联合顺铂对卵巢癌细胞COC1的体外抗肿瘤作用

利用MTT比色法研究了姜黄素(Cur)联合顺铂（DDP）对卵巢癌COC1细胞的抑制作用。 结果表明，当ρ(DDP)为2.5~10 mg/L，ρ(Cur)=5 mg/L时，与DDP相比，二者联合用药对卵巢癌COC1细胞可产生协同抑制作用；ρ(Cur)=5 mg/L就可明显增强COC1细胞对顺铂的敏感性，增敏倍数为2.66倍；光学显微镜观察发现，药物作用后COC1细胞的形态发生了改变，细胞折光度下降，大小不一、形态各异、排列疏散和胞浆空泡化严重。 为探讨姜黄素协同顺铂抑制肿瘤的机制，本研究采用高效液相色谱法测定姜黄素辅助用药前后细胞内DDP含量的变化。 结果表明，在加入姜黄素前后，COC1细胞内顺铂含量虽然略有下降，但差异无统计学意义（P＞0.05）。 由此可见，姜黄素与顺铂联合用药对人卵巢癌COC1细胞增殖具有协同抑制作用，且姜黄素可同时增加COC1细胞对顺铂的敏感性，但姜黄素可能并未明显改变顺铂在细胞内的含量，即姜黄素可能不是通过影响顺铂进入细胞的通路而起协同抑制作用的。

Anti-tumor Effect of Curcumin Combining with Cis-Platin on Human Ovrian Cancer Cell Line COC1 in vitro

Inhibiting effect of the curcumin(Cur) in combination with cis- diamminedichloroplatinum（DDP） on proliferation of COC1 cells was analyzed with MTT colorimetric assay. The morphological changes of COC1 cells were observed by inverted microscope. The result indicated that DDP of 2.5～10 mg/L dose combined with curcumin(Cur) of 5 mg/L dose could enhance the inhibiting effect and induce apoptosis compared with the case when only DDP was used. Meanwhile, curcumin of 5 mg/L dose could improve the sensitization of COC1 cells to DDP with 2.66 times. Optical microscopy results showed that COC1 cells had a marked decreased proliferation and a great amount of cells necrosis in DDP+Cur group and DDP group than the control group. COC1 cells had lower diopter, scatter array, serious vacuolization treated with DDP+Cur. The HPLC result indicated that the contents of DDP within tumor cells decreased lightly after dosage of Cur, but it had no statistically significant(P＞0.05). In summary, curcumin combine with DDP can enhance the inhibiting effect on proliferation of COC1 cells, meanwhile, curcumin can improve the sensitization of COC1 cells to chemotherapeutics. However, the curcumin is unlikely to affect the amount of DDP within cells.