一种表征羟基自由基的新型荧光探针

表征羟基自由基 (· OH)的方法主要有电子自旋共振法1] 和芳环羟基化法2 ,3] 两大类 .电子自旋共振法灵敏度不高 ,且仪器设备昂贵 ,不适于常规分析 .芳环羟基化法操作较简单 ,灵敏度高 ,但芳环羟基化的产物往往不止一种 ,使得定量测定变得复杂 .其它方法如高效液相色谱法4 ] ,化学发光法5] 等也有报道 .顺磁性氮氧化合物能有效地清除自由基 6 ,7] ,同时也是一种芳烃单重激发态的有效猝灭剂 8,9] .当顺磁性氮氧化合物与荧光分子共价结合 ,所形成的荧光分子 -氮氧自由基复合物 (即自旋标记荧光分子 )仍保留对自由基反应的活性 ,但由于…

A Novel Fluorescence Probe for the Determination of Hydroxyl Radicals

A novel technique for the determination of the hydroxyl radical is described in this paper. The method employed the reaction of hydroxyl radical with dimethyl sulfoxide to generate a methyl radical quantitatively, which then coupled with the nitroxide linked naphthalene and led to a large increase in fluorescence intensity. The proposed method was demonstrated to be capable of detecting hydroxyl radicals generated in the Fenton systems. It proved to be a simple and sensitive technique for the determination of hydroxyl radicals.