Preparation of enzyme multilayers on colloids for biocatalysis

The construction of enzyme multilayer films on colloidal particles for biocatalysis is described. The enzyme multilayers were assembled on submicrometer‐sized polystyrene spheres via the alternate adsorption of poly(ethyleneimine) and glucose oxidase using a layer‐by‐layer approach. Microelectrophoresis and single particle light scattering measurements revealed regular and step‐wise assembly of the multilayers on the colloids. The high surface area bio‐multilayer coated particles formed were subsequently utilized in enzymatic catalysis.