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Evaluation of labelling conditions,quality control and biodistribution study of 99mTc-5-aminolevulinic acid (5-ALA): a potential liver imaging agent
Authors:Kalim Ullah Khan  Samina Roohi  Mohammad Rafi  Rizwana Zahoor  Zafar Iqbal  Mushtaq Ahmad
Affiliation:1. Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan
2. Isotope Production Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, 45650, Pakistan
Abstract:Labelling of 5-aminolevulinic acid (5-ALA) with 99mTc was achieved by using SnCl2·2H2O as reducing agent. Radiochemical purity and labelling efficiency was determined by instant thin layer chromatography/paper chromatography. Efficiency of labelling was dependent on many parameters such as amount of ligand, reducing agent, pH, and time of incubation. 99mTc labelled 5-ALA remained stable for 24 h in human serum. Tissue biodistribution of 99mTc-5-ALA was evaluated in Sprague–Dawley rats. Biodistribution study (% ID/g) in rats revealed that 99mTc-5-ALA was accumulated significantly in liver, spleen, stomach and intestine after half hour, 4 and 24 h. Significant activity was noted in bladder and urine at 4 h. High liver uptake of 99mTc-5-ALA makes it a promising liver imaging agent.
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