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Hyperfine Interaction Tensors of 13C Nuclei for Ring Carbons of Ubisemiquinone-10 Hydrogen Bonded in Alcohol Solvents |
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| Authors: | Rimma I Samoilova Alexander T Taguchi Patrick J O’Malley Sergei A Dikanov Johan Lugtenburg |
| Institution: | 1. V. V. Voevodsky Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk, 630090, Russian Federation 2. Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA 4. School of Chemistry, The University of Manchester, Manchester, M13 9PL, UK 3. Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA 5. Leiden Institute of Chemistry, Gorlaeus Laboratory, Leiden University, P. O. Box 9502, 2300 RA, Leiden, Netherlands |
| Abstract: | The anion radicals of ubiquinones-10 13C chemically labeled at the C5 or C6 ring positions in alcohol have been studied by 1D and 2D ESEEM to define the hyperfine interaction tensors with the 13C nuclei. Analysis of the cross-peak line shapes and simulations of the spectra allowed us to conclude that the hyperfine tensors are characterized by an anisotropic component T ~6 MHz and an isotropic coupling a ~?3 MHz with support from DFT calculations. However, these values were found to be inconsistent with the shift of the sum combination harmonic in the four-pulse ESEEM spectra. Simulations resolve this apparent discrepancy by showing that the shift of the sum combination to lower frequency and its broadening can be accounted for by a distribution of the hyperfine couplings. A spread of the methoxy group conformations, as supported by previous experimental observations, is suggested as the mechanism influencing the distribution of the hyperfine couplings for the ring carbons. |
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