| 水产品中螺旋霉素、替米考星、泰乐菌素与北里霉素残留量的超高效液相色谱-紫外检测法同时测定 |
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| 引用本文： | 刘永涛,艾晓辉,邹世平,杨红.水产品中螺旋霉素、替米考星、泰乐菌素与北里霉素残留量的超高效液相色谱-紫外检测法同时测定[J].分析测试学报,2010,29(3). |
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| 作者姓名： | 刘永涛 艾晓辉 邹世平 杨红 |
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| 作者单位： | 中国水产科学研究院,淡水渔业研究中心,江苏,无锡,214081;中国水产科学研究院长江水产研究所,农业部淡水鱼类种质监督检验测试中心,湖北,荆州,434000 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目 |
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| 摘 要： | 建立了水产品肌肉组织中螺旋霉素、替米考星、泰乐菌素、北里霉素同时测定的超高效液相色谱-紫外检测(UPLC-TUV)方法。样品经乙腈提取后,浓缩至近干,用4%NaCl溶解残渣,正己烷除脂,经固相萃取小柱净化,乙腈洗脱;以乙腈-25 mmol/L磷酸二氢铵(pH2.5,含10%乙腈)为流动相,以ACQUITYUPLC BEHC18为分离柱,柱温为45℃,流速为0.3 mL/min,紫外检测。方法在0.100~20.0 mg/L范围内呈线性相关,螺旋霉素、替米考星、泰乐菌素和北里霉素的相关系数分别为0.998 7、0.999 3、0.999 4和0.998 0。平均回收率为70%~102%,相对标准偏差为2.9%~11.2%,螺旋霉素、替米考星、泰乐菌素和北里霉素的检出限分别为25、25、50、75μg/kg。方法满足水产品肌肉组织中螺旋霉素、替米考星、泰乐菌素和北里霉素的残留量测定。
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| 关 键 词： | 水产品 螺旋霉素 替米考星 泰乐菌素 北里霉素 残留量 超高效液相色谱 |
Simultaneous Determination of Spiramycin,Tilmicosin,Tylosin and Kitasamycin Residues in Fishery Products by UPLC-TUV Method |
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| LIU Yong-tao,AI Xiao-hui,ZOU Shi-ping,YANG Hong.Simultaneous Determination of Spiramycin,Tilmicosin,Tylosin and Kitasamycin Residues in Fishery Products by UPLC-TUV Method[J].Journal of Instrumental Analysis,2010,29(3). |
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| Authors: | LIU Yong-tao AI Xiao-hui ZOU Shi-ping YANG Hong |
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| Institution: | LIU Yong-tao1,2,AI Xiao-hui1,ZOU Shi-ping1,YANG Hong1,2(1.Freshwater Fisheries Research Center,Chinese Academy of Fishery Science,Wuxi 214081,China,2.Freshwater Fish Germplasm Quality Supervision , Testing Center,Ministry of Agriculture,Yangtze River Fisheries Research Institute,Jingzhou 434000,China) |
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| Abstract: | An ultra high performance liquid chromatographic(UPLC) method for simultaneous determination of residues of spiramycin(SPM),tilmicosin(TIL),tylosin(TYL) and kitasamycin(KIT) in fishery products has been developed.The muscle tissue sample was extracted by acetonitrile,and the extracts were evaporated to near dryness and redissolved in 4% NaCl water solution.The extracts were defatted with hexane,and cleaned up on a HLB SPE column.The analytes was separated on ACQUITY UPLC BEH C18 with acetonitrile-25 mmol/L ... |
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| Keywords: | fishery pruducts spiramycin tilmicosin tylosin kitasamycin residues ultra high performance liquid chromatography(UPLC) |
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