Gamma irradiation of sorghum flour: Effects on microbial inactivation, amylase activity, fermentability, viscosity and starch granule structure |
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Authors: | Ivan M Mukisa Charles MBK MuyanjaYusuf B Byaruhanga Reidar B SchüllerThor Langsrud Judith A Narvhus |
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Institution: | a Department of Chemistry, Biotechnology and Food Science (IKBM), Norwegian University of Life Sciences (UMB), P.O. Box 5003, NO-1432 Ås, Norway b Department of Food Technology and Nutrition, Makerere University, P.O. Box 7062, Kampala, Uganda |
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Abstract: | Malted and un-malted sorghum (Sorghum bicolor (L.) Moench) flour was gamma irradiated with a dose of 10 kGy and then re-irradiated with 25 kGy. The effects of irradiation on microbial decontamination, amylase activity, fermentability (using an amylolytic L. plantarum MNC 21 strain), starch granule structure and viscosity were determined. Standard methods were used during determinations. The 10 kGy dose had no effect on microbial load of un-malted flour but reduced that of malted flour by 3 log cycles. Re-irradiation resulted in complete decontamination. Irradiation of malt caused a significant (p<0.05) reduction in alpha and beta amylase activity (22% and 32%, respectively). Irradiation of un-malted flour increased the rates of utilization of glucose and maltose by 53% and 100%, respectively, during fermentation. However, microbial growth, rate of lactic acid production, final lactic acid concentration and pH were not affected. Starch granules appeared normal externally even after re-irradiation, however, granules ruptured and dissolved easily after hydration and gelatinization. Production of high dry matter density porridge (200 g dry matter/L) with a viscosity of 3500 cP was achieved by irradiation of un-malted flout at 10 kGy. Gamma irradiation can be used to decontaminate flours and could be utilized to produce weaning porridge from sorghum. |
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Keywords: | Sorghum Starch Irradiation Viscosity Fermentability |
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